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The effects of different tocopherols on HMG-CoA mRNA mRNA of HepG2 cells. Cells were incubated for 24 h at 37°C in media containing the indicated concentrations of α-tocopherol. HMG-CoA mRNA was measured using a PCR and western blotting technique as described in Methods and materials and the data was expressed as the percent difference from the values obtained with control cells not pretreated with α-tocopherol. The HMG-CoA mRNA in control cells averaged 4.2 ± 0.8 × 105 copies/μg RNA.
