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. 2003 May 9;2:12. doi: 10.1186/1475-2875-2-12

Table 1.

Sequences of oligonucleotide primers used to amplify merozoite surface protein (msp)-1 and msp-2 polymorphic regions of Plasmodium falciparum isolates from Gabon.

Gene Primer Sequence Notes
msp1-C1 5'-AAGCTTTAGAAGATGCAGTATTGAC Conserved
msp1-C2 5'-ATTCATTAATTTCTTCATATCCATC Conserved
K1a 5'-GAAATTACTACAAAAGGTGCAAGTG K1 family-specific
msp-1 K1b 5'-AGATGAAGTATTTGAACGAGGTAAAGTG K1 family-specific
MAD20a 5'-AAATGAAGGAACAAGTGGAACAGCTGTTAC MAD20 family-specific
MAD20b 5'-ATCTGAAGGATTTGTACGTCTTGAATTACC MAD20 family-specific
RO33a 5'-TAAAGGATGGAGCAAATACTCAAGTTGTTG RO33 family-specific
RO33b 5'-CATCTGAAGGATTTGCAGCACCTGGAGATC RO33 family-specific
msp2-C1 5'-ATGAAGGTAATTAAAACATTGTCTATTATA Conserved
msp2-C2 5'-CTTTGTTACCATCGGTACATTCTT Conserved
msp-2 FC27a 5'-GCAAATGAAGGTTCTAATACTAATAG FC27 family-specific
FC27b 5'-GCTTTGGGTCCTTCTTCAGTTGATTC FC27 family-specific
3D7a 5'-AGAAGTATGGCAGAAAGTAAGCCTCCTACT 3D7 family-specific
3D7b 5'-GATTGTAATTCGGGGGATTCAGTTTGTTCG 3D7 family-specific