Table 4.

Primers used in PCR and RT-PCR experiments performed in this study Primer sequences are shown 5' to 3'. "L" annotates sense, "R" antisense primers. In addition, the proposed gene names, as depicted in Fig. 1, and PCR product sizes are shown (see also Fig. 5).

Synechocystis PCC 6803	Primer Sequence	Gene Name	Product Size [bp]
Bidirectional hydrogenase
hoxH6803/L	AAT CCC ACG CCC TAA GTT TT
		hoxH	200
hoxH6803/R	CAC TGA CCA AGC AGA GTG GA
hoxW6803/L	TCA TCG GTT ACG GCA ATA CC
		hoxW	198
hoxW6803/R	ATG GTT CGT TTG CTG ATT CC
Nostoc punctiforme ATCC 29133/PCC 73102
Uptake hydrogenase
hupL-L	CTG TTG GGC GGA CAA TGG CCT CA
		hupL	383
hupL-R	CCA CTT TTC ATA ATC AT
hybD73102/L	CGC AGG GAT GGA AGT AAT GT
		hupW	218
hybD73102/R	TCA CAT CAT CGG GAA AGT CA
Anabaena PCC 7120
Uptake hydrogenase
hupL7120/L2	TAT ATC ACC CGT CGG TAG AG
		hupL	205
hupL7120/R2	CCA TGA TGC AGA GGT TAA GT
hybD7120/L	CGC AGG GAT GGA AGT TAT GT
		hupW	209
hybD7120/R	GCG GAA AAT CAT CTG GAA A
Bidirectional hydrogenase
hoxHF	CCA CTA TGC TCG TTT AAT TGA AAT
		hoxH	355
hoxHR	GGA ACA ACT TAA ACA GGG GTC AAA
hoxW7120/L2	ATC TCT TGC AGT CCA TCA AC
		hoxW	200
hoxW7120/R2	CGT AGA TGG CTT TGG TTA AG