Figure 3.

Interaction of recombinant N and P proteins with profilin ex vivo. A549 cells were transfected with varying amounts of pcDNA3 clone of N or P, and a representative set of results is shown. Lysis and immunoprecipitation (IP) of the transfected cells were carried out as described in Materials and Methods. The resultant precipitate was probed in immunoblot (IB). The antibodies were against N, P, or profilin (Pr), as indicated. Lane 0 indicates that IP was performed with protein A-sepharose only without any antibody. The N, P, and profilin bands are so marked.