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Requirement of profilin in RSV-induced cell fusion. Photomicrographs of HEp-2 and A549 monolayers were taken at 48 h post-infection as described under Materials and Methods. siRNA indicates pre-treatment with anti-profilin siRNA. Note the fused cells (syncytia) in panel C, and cytopathic effect (sick, rounded cells) but no syncytia in panel D. Also note that the siRNA alone had no discernible effect on cell morphology.
