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. 2003 Jun 1;31(11):2900–2914. doi: 10.1093/nar/gkg380

Figure 6.

Figure 6

The N-terminal region of ZBP-89 is required for repression. (A) A schematic of ZBP-89 and its various deletion mutants. The amino acid position of each deletion is noted. (B) The various ZBP-89 deletion mutants (gray boxes) in the pPac vector (1 µg) were co-transfected with pPacSp1 (0.2 µg) and 353WT (5 µg) into S2 cells. Transfection of pPacSp1 alone is indicated by the black box. CAT activity is expressed as the mean ± SE. (C) Western blot analysis of WCEs prepared from S2 cells transfected with pPacZBP-89 and its various deletion mutants (HA-tagged) incubated with anti-HA antibody as follows: lane 1, non-transfected control; lane 2, transfected with pPacZBP-89-HA; lane 3, transfected with dA; lane 4, transfected with dAB1; lane 5, transfected with dB2C; lane 6, transfected with dS; lane 7, transfected with dSC; lane 8, transfected with dC. The position of migration of molecular weight markers is noted.