Figure 7.

Human TAF_II130 can reverse the repression of ZBP-89 on Sp1. (A) Increasing amounts (0.5 and 2 µg) of pPac-hTAF_II130 were co- transfected with 353WT (5 µg), pPacSp1 (0.2 µg) and pPacZBP-89 (1 µg) into S2 cells (black box). A second set of transfections replaced pPacSp1 with the Sp1 mutant 516c-Δint266 lacking both glutamine-rich domains (gray box). CAT activity is expressed as the mean ± SE. (B) Western blot analysis of WCEs prepared from S2 cells non-transfected (blank) or transfected with pPac-hTAF_II130 (myc-tagged) and incubated with anti-Myc antibody. The position of migration of molecular weight markers is noted.