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Reporter plasmids for functional INS analysis. The construct pLRedR contains the HIV 5′-LTR as promoter/enhancer, an ORF encoding a red fluorescent reporter protein (RFP; DsRed1), multiple additional translational STOP codons for termination of the DsRed ORF, the RRE and the 3′-LTR of HIV for termination of transcription. Different INS regions (Fig. 4) were inserted in sense and antisense orientation into the unique ClaI restriction site of the basic reporter plasmid pLRedR.
