Figure 8.

Rev rescue of INS inhibitory activities. To analyze whether the inhibitory activity of known and predicted INS regions of the HIV-1 genome can be overcome by HIV-1 Rev protein, reporter plasmids with the indicated INS regions were cotransfected in parallel with either pCsRevsg143 for expression in the presence of Rev-GFP or pFRED143 for expression in the absence of Rev. Reporter plasmid pLRedR, which lacks INS (Fig. 5), was analyzed as a control. Cells showing green and red fluorescence were counted and the Rev-induced increase in the number of dual-fluorescent cells determined (detailed in Materials and Methods). The effect of Rev on all INS regions was analyzed in sense (black bars, s) and antisense orientations (gray bars, as).