Table 1. Oligonucleotide primer positions and product lengths.
| Target | Region | Forward primer | Reverse primer | Length of PCR product (bp) |
|---|---|---|---|---|
| mtDNAa | A | L3108(20) | H3500(20) | 393 |
| B | L5223(24) | H5586(20) | 364 | |
| C | L12759(21) | H13188(22) | 430 | |
| D | L14820(23) | H15253(20) | 434 | |
| β-Globinb | (5′) 84–103 (3′) | (5′) 439–421 (3′) | 356 |
aNomenclature of primers is according to Vaillant and Nagley (19). The first number after L or H (corresponding to light and heavy chains of human mtDNA, respectively) is the sequence position (20) of the 5′ end of the primer. The number in parentheses is the length of the primer in nucleotides. Each primer pair corresponds to a particular region of mtDNA used for quantification of total mtDNA.
bSequence as reported in Lawn et al. (21), with nucleotide positions relative to 5′ end of first exon of β-globin.