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. 1994 Sep;102(Suppl 3):227–229. doi: 10.1289/ehp.94102s3227

Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.

J Chen 1, W G Thilly 1
PMCID: PMC1567402  PMID: 7843103

Abstract

A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure mutant frequencies as a function of DNA sequence. Chromium(VI) induced four hotspots in a 104 bp domain of hprt exon 3. Substitutions at G:C base pairs were the predominant mutations. One of the chromium-induced hotspots was located at the same position as previously determined hydrogen peroxide and benzo(a)pyrene diol epoxide hotspots.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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