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Selective release of poly(A)+ RNA from the cytoplasm by digitonin permeabilization. In situ hybridizations were performed on intact cells (– digitonin) or permeabilized cells (+ digitonin). Nuclei were visualized by Hoechst staining (left). Some cells with a strong cytoplasmic staining for poly(A)+ RNA (– digitonin; right) are marked by arrows. Bar 20 µm.
