Figure 5.
Nuclear export in vitro does not depend on exogenous cytosol or ATP. (a) Nuclear export reactions were performed at 4 or 25°C with or without cytosol (1 mg/ml), as indicated. The dotted line represents a reaction at 25°C with cytosol but without cells. (b) Reactions were performed in the presence of an ATP-regenerating system (solid lines) or an ATP-depleting system (dotted lines) at either 4 or 25°C, as indicated.