Fig. 5. Chromatin immunoprecipitation of AML1 in Kasumi-1, HL60, HL60-PMA and HeLa cells as well as cells from leukaemia patients with or without a t(8;21) with quantification of recovered DNA at eight points along the c-FMS locus. The locations of the promoter, FIRE –1 kb and FIRE regions are indicated (P, F-1kb and F), and DNA was quantified as in Materials and methods. With Kasumi-1 chromatin and chromatin from primary t(8;21) cells, the AML1 antibody significantly enriches the DNA from the intronic regulatory elements with very little promoter enrichment. Precipitation of HL60 and HL60-PMA chromatin with an AML1 antibody enriched both promoter and intronic DNA although amounts precipitated were significantly less than seen with Kasumi-1 chromatin. No specific DNA enrichment was seen with HeLa chromatin.