Fig. 7. Chromatin immunoprecipitation of (A) acetylated Lys9 of histone H3 and (B) methylated Lys9 of histone H3 in Kasumi-1, HL60, HL60-PMA and HeLa cells, with quantification of recovered DNA at eight points along the c-FMS locus. The locations of the promoter, FIRE –1 kb and FIRE regions are indicated (P, F-1kb and F), and DNA was quantified as in Materials and methods. Differentiation of HL60 cells with PMA induces a marked increase in acetylation up- and downstream of FIRE, while FIRE remains relatively deacetylated. Kasumi-1 H3 is acetylated either side of FIRE although relatively less than HL60-PMA chromatin. HeLa H3 is not acetylated along this locus. Both HL60 and HeLa H3 are methylated at K9 across the locus, while PMA differentiation of HL60 cells induces general demethylation, most markedly over FIRE. Kasumi-1 histone H3 (K9) appears relatively methylated, with some reduction over the intronic regulatory region.