Fig. 3. Comparison of phenotypes of original transposon insertion mutants with those of reconstructed hemizygous deletion mutants. In each case, the deletion mutant was constructed in a fresh strain (RM1000) by deleting one copy of the indicated gene. These genes were identified by the site of transposon insertion in the original mutants. Colonies were grown on the indicated media for the indicated length of time and photographed at 2.5× magnification. Note the complex and individualistic colony morphologies, particularly those of the ZAP1/zap1 and FGR22/fgr22 mutants.