Fig. 6. Overproduced Gsk3 kinase suppresses mis12 and antagonizes Ppe1. (A) Multicopy plasmid pGsk3 suppressed the ts phenotype of mis12-537 at 36°C, but did not suppress cnp1-1 and mis6-302 mutants even at 33°C. (B) Gsk3 is antagonistic to Ppe1 and Ekc1, as its moderate overexpression by REP41 plasmid in the absence of thiamine prevented colony formation of ekc1-163 and Δppe1, but not of wild-type. Even the milder expression of Gsk3 by REP81 plasmid still caused retarded growth of ekc1 and Δppe1 mutants. REP1 caused the strongest overexpression. (C) Gsk3 overexpression in the wild-type cells led to the accumulation of pear-shaped cells. Wild-type cells in which Gsk3 was expressed by REP1 plasmid in the absence of thiamine for 24 h at 26°C. Cells were stained by DAPI. Bar, 10 µm.