Fig. 3.

Imaging methodology for quantifying recombinant cells in whole pancreata. (a) Compiled image of 9-week-old FYDR pancreas at ×1 taken under visible light (Left) and an EYFP filter (510–560 nm) (Right). (Scale bar, 1 cm.) The edge of the pancreatic tissue is outlined. (b) Method for reducing background fluorescence. (Upper) Background fluorescence appears brightly under EYFP (510–560 nm, column 1) and red (605/55 nm, column 2) filters. The red-filtered image is inverted (column 3) and merged with the EYFP-filtered image (column 4). (Lower) Similar analysis of an EYFP focus.