Fig. 5.
Effects of aging on the frequency of recombinant cells in the pancreas. (a) Compiled images for juvenile (4 weeks old) (Left), adult (9 weeks old) (Center), and aged (64–72 weeks old) (Right) FYDR mice. Examples of pancreata with the highest number of recombinant foci for each cohort are shown. Images were collected at ×1 with an EYFP filter (510–560 nm). (Scale bar, 1 cm.) The edge of the pancreatic tissue is outlined. (b) Recombinant foci per pancreas detected by in situ image analysis for juvenile (n = 25), adult (n = 24), and aged (n = 16) mice. (c) Weight (mg) and area (cm2) of mouse pancreata for juvenile (n = 25), adult (n = 25), and aged (n = 17) cohorts. (d) Recombinant foci per squared centimeter for juvenile (n = 25), adult (n = 24), and aged (n = 16) mice. (e) Frequency of recombinant cells per million as determined by flow cytometry for juvenile (n = 24), adult (n = 24), and aged (n = 16) mice. (b–e) ∗, adult cohort is significantly higher statistically than juvenile cohort (P < 0.05). ∗∗, aged cohort is significantly higher statistically than juvenile and adult (P < 0.05) cohorts. Medians are indicated by black bars. Points on the x axis indicate individual mice with zero recombinant cells detected. (f) Images of the five largest foci among all juvenile (Left) and all aged (Right) mice taken under an EYFP filter (510–560 nm) at ×1. (Scale bar, 100 μm.) Brightness and contrast were not adjusted.