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. 2006 Jul 31;103(32):11874–11879. doi: 10.1073/pnas.0605303103

Fig. 2.

Fig. 2.

Two distinct POT1-containing protein complexes exist in human cells. (a) HeLa cell nuclear extracts (HeLa NE) were fractionated on a DEAE ion-exchange column. The bound proteins were eluted with increasing concentrations of KCl. The two main fractions (I and II) that contained telomeric proteins were then further fractionated on a gel filtration column. (b) Fractions from the DEAE column were collected and resolved by SDS/PAGE. Telomeric proteins were detected through Western blotting by using various antibodies as indicated. “Flow” indicates flow through. (c) Fraction I from the DEAE column was fractionated on a gel filtration column. Telomeric proteins at individual fractions (fractions 18–31) were detected through Western blotting with different antibodies as indicated. The majority of telomeric proteins were coeluted at ≈500 kDa (indicated by the box). Molecular masses of the gel-filtration standards are indicated.