Table 1.
Kinetic parameters for residues of Groups 1, 2, and 3
| Group | χ2/N | 12°C |
17°C |
22°C |
27°C |
||||
|---|---|---|---|---|---|---|---|---|---|
| ka, s−1 | kb, s−1 | ka, s−1 | kb, s−1 | ka, s−1 | kb, s−1 | ka, s−1 |
kb, s−1 |
||
| 1 | 0.6 | — | — | 5.7 ± 0.3 | 751 ± 76 | 9.9 ± 0.4 | 1,267 ± 56 | 16.6 ± 0.9 | 1,983 ± 102 |
| 2 | 0.9 | 21.9 ± 0.3 | 119 ± 16 | 35.1 ± 0.6 | 200 ± 11 | 60 ± 2 | 361 ± 10 | 94 ± 3 | 555 ± 10 |
| 3 | 1.0 | 80 ± 4 | 387 ± 12 | 106 ± 5 | 592 ± 9 | 155 ± 7 | 1,008 ± 16 | 193 ± 12 | 1,460 ± 35 |
Group 1 consists of 15N data of amides from Val-7, Arg-79, Ile-80, Lys-82, Asp-83, and His-84 and 13C data of methyls from Val-7, Val-76, and Ile-80. Group 2 consists of 15N amide dispersions from His-92, Glu-94, Trp-96, Leu-97, Arg-98, Phe-225, and Nε1 from Trp-96. Group 3 includes amide dispersions from Ala-12, Ile-13, Thr-14, Val-17, His-63, Gly-138, His-144, Tyr-146, Glu-168, Thr-178, Ala-184, Ala-200, Gly-201, and Met-205 and methyl dispersions from Val-17, Val-166, Val-181, Leu-182, Val-185 (2), Leu-186, and Leu-212. None of the 15N data recorded at 12°C are used for Group 1 because the dispersions are very small. χ2/N is the reduced χ2 value for the fit. (See Materials and Methods for details on fitting.)