Table 1.
Comparison of results for each protein fragment
| Sample | MW, kDa | MALLS Rh, nm | Guinier Rg, nm | GNOM Rg, nm | Dmax, nm | Rod Dmax, nm | Rod, % | Rod Rg, nm | Rod χ |
|---|---|---|---|---|---|---|---|---|---|
| PF2 | 42.7 | 4.3 | 4.3 | 4.7 | 15.0 | N/D | N/D | N/D | N/D |
| PF5 | 37.8 | 3.8 | 3.6 | 3.9 | 11.5 | 14.6 | 79 | 4.1 | 2.65 |
| PF7 | 36.4 | 3.3 | 4.0 | 4.3 | 13.0 | 17.8 | 73 | 5.6 | 5.16 |
| PF8 | 39.1 | 3.8 | 4.2 | 4.5 | 14.0 | 20.5 | 68 | 6.3 | 6.77 |
| PF9 | 29.7 | 3.6 | 3.3 | 3.2 | 9.0 | 10.7 | 84 | 3.4 | 7.71 |
| PF10 | 52.6 | N/D | 3.6 | 3.8 | 11.0 | 23.4 | 47 | 7.7 | 9.42 |
| PF11 | 83.2 | 5.8 | 4.8 | 4.4 | 13.5 | 31.3 | 43 | 9.5 | 8.60 |
| PF12 | 42.7 | 3.9 | 3.8 | 4.1 | 12.5 | 17.2 | 73 | 5.6 | 5.52 |
| PF13 | 43.0 | N/D | 3.9 | 4.3 | 12.2 | 20.5 | 60 | 6.3 | 7.77 |
The following data are provided for each protein fragment: the predicted molecular mass (MW; calculated from the primary sequence); the hydrodynamic radius (Rh) from MALLS; the Rg from the Guinier approximation and GNOM; the Dmax; the measured maximum dimension of a rod-shaped model, i.e., a linear array of domains (Rod Dmax); the experimental Dmax/Rod Dmax expressed as a percentage (% Rod); the theoretical Rg of the rod model (Rod Rg); and the discrepancy factor χ between the rod model and experimental data (Rod χ). N/D, not determined.