Fig. 2.

Structural organization of WDFY2 and conservation of functional motifs. C. elegans WDFY2 (cWDFY2) and the human homolog (hWDFY2) consist of seven WD40 repeats with a FYVE domain inserted between repeats WD6 and WD7. The WD40 repeats are predicted to form a b propeller structure with seven blades. The predicted site of insertion in the loop connecting repeats WD6 and WD7 of human WDFY2 is indicated with respect to the structure of UNC-78 (Protein Data Bank ID code 1PEV), which represents the closest structural homolog identified by threading by using the Phyre fold recognition server. The FYVE domain of EEA1 (Protein Data Bank ID code 1JOC) is depicted on the right. Note that the FYVE domains of WDFY2 and FENS-1 contain a large insertion at the N terminus of the membrane insertion loop. Structural images were rendered with PyMol (www.pymol.org).