Fig. 7.

Inhibition of Tf uptake by silencing of WDFY2. HeLa cells were treated with silencing oligonucleotides to the proteins indicated. (A) Cells were incubated for 5 min with fluorescent Tf, acid-washed to remove noninternalized Tf, fixed, counterstained with DAPI, and visualized. (Magnification: ×200.) (B) Cells were incubated for 5 or 15 min with Tf, acid-washed, and lysed, and total fluorescence in the lysate was quantified. Plotted are the means and SEM of three independent experiments performed in triplicate. (C) Western blots of cell lysates stained with antibodies to the Tf receptor (anti-TfR) or WDFY2.