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. 2006 Aug 1;103(32):11946–11951. doi: 10.1073/pnas.0510138103

Fig. 4.

Fig. 4.

ANP expression is increased in AKT-TAC relative to NTG-TAC mice at 1 wk after TAC surgery. (a) Transcript levels for ANP, BNP, c-fos, β-MHC, and α-SKA were quantified by real-time RT-PCR in mRNA samples. Fold change in abundance is presented after standardization to GAPDH as an internal control. ANP mRNA transcript levels are significantly increased in AKT-TAC (gray bars) relative to NTG-TAC (black bars) samples (∗∗, P < 0.01). In contrast, transcript levels of BNP and α-SKA were significantly decreased in AKT-TAC relative to NTG-TAC samples (∗∗, P < 0.01; ∗, P < 0.05). (b) Changes in mRNA transcript levels represented as fold change in sham-operated AKT relative to NTG myocardial samples probed for ANP, BNP, c-fos, β-MHC, and α-SKA by real-time RT-PCR. Fold change in abundance is presented after standardization to GAPDH as an internal control. No significant differences were observed between AKT and NTG samples before TAC challenge. (c) Cardiac lysates at 1 wk after surgery were examined for protein expression of ANP, myc-tag, and Akt by immunoblot analyses with GAPDH and were included to standardize for minor variations in loading between samples. (d) Quantitative densitometry of ANP relative to GAPDH protein levels in NTG-sham, NTG-TAC, AKT-sham, and AKT-TAC mice.