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. 2006 Aug 1;103(32):11952–11957. doi: 10.1073/pnas.0601931103

Fig. 3.

Fig. 3.

Altered levels of O-GlcNAc correlate with changes in phosphoprotein profiles. Shown is a comparison of lysates from WT (N2), oga-1(ok1207), and ogt-1(ok430) animals. Samples were resolved by SDS/PAGE and examined by Western blot analysis. (A) Blots were probed with a monoclonal antibody specific for O-GlcNAc-modified nucleoporins (RL2) and a monoclonal antibody specific for nucleoporins (RL1). Molecular mass standards are shown on the left. Altered migration because of the absence of O-GlcNAc on p82 is indicated by the arrowhead. (B) Phospho-serine (Anti-P-Ser) and phospho-threonine (Anti-P-Thr) antibodies were also used to detect differences in phosphorylation between the strains. Asterisks show migration of three proteins (p60, p12, and p6) with changes in apparent phosphorylation levels. In control experiments, phosphatase treatment reduced the observed levels of Ser- and Thr-phosphorylation by 70% and 66%, respectively. (C) Elevation of GSK protein (Left) and phospho-GSK (Right) levels in the mutant strains are shown. Data are representative of three separate experiments.