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Codepletion of the spindle assembly checkpoint proteins relieves Chk1 depletion-induced mitotic arrest. (A) BubR1 or Mad2 was codepleted with Chk1 in synchronized HeLa cells, as described in the text. Fourteen hours after release from the double thymidine block, cells were harvested, lysed, and analyzed with Western blotting using the antibodies indicated. (B) Fourteen hours after release from the block, Chk1/BubR1- or Chk1/Mad2-codepleted cells were stained with phospho-H3 antibody.
