. 2006 Jul 28;103(32):11975–11980. doi: 10.1073/pnas.0605087103

Table 1.

Transcription of spermatogenesis-related genes in testes of the male sterile mutants

Transcript	Amount of transcript in mutant testes compared with wild type (SD)				Fold down-regulation in the modulo mutant (probability of value being equal to 1.0)
Transcript	achi/vis	rye	sa	mod
mod	1.425 (0.000)	0.919 (0.546)	1.391 (0.247)	0.177 (0.097)	5.7 (0.005)^*
Ubiquitously expressed genes
Act5C	0.267 (0.003)	0.415 (0.008)	0.408 (0.042)	1.533 (0.746)	0.7 (0.34)
Rpl9	1.071 (0.079)	0.665 (0.085)	1.082 (0.064)	0.966 (0.205)	1.0 (0.8)
Broadly expressed spermatogenesis-related genes
twe	0.281 (0.018)	0.352 (0.142)	0.611 (0.104)	0.674 (0.356)	1.5 (0.25)
des	0.503 (0.022)	0.835 (0.012)	1.049 (0.285)	0.656 (0.306)	1.5 (0.19)
Meiotic arrest genes
aly	0.324 (0.047)	0.223 (0.023)	0.327 (0.050)	0.205 (0.117)	4.9 (0.007)^*
ach/vis	0.344 (0.050)	0.360 (0.067)	0.238 (0.026)	0.541 (0.483)	1.8 (0.24)
can	0.312 (0.014)	0.334 (0.051)	0.583 (0.000)	0.162 (0.174)	6.2 (0.014)^†
mia	0.286 (0.010)	0.257 (0.070)	0.363 (0.037)	0.459 (0.655)	2.2 (0.29)
rye	0.687 (0.003)	0.065 (0.024)	2.137 (0.188)	0.135 (0.147)	7.4 (0.001)^*
nht	0.234 (0.026)	0.437 (0.015)	0.482 (0.092)	0.135 (0.132)	7.4 (0.001)^*
Testes-biased genes relatively insensitive to mutations in meiotic arrest genes and modulo
CG13981	0.554 (0.135)	0.309 (0.008)	0.215 (0.101)	0.840 (0.085)	1.2 (0.082)^*
Crtp	0.175 (0.052)	0.229 (0.040)	0.181 (0.018)	0.352 (0.130)	2.8 (0.013)^†
Ku80	1.432 (0.035)	0.826 (0.093)	1.356 (0.100)	1.255 (0.316)	0.8 (0.30)
Yu	0.199 (0.062)	0.178 (0.041)	0.295 (0.100)	0.591 (0.268)	1.7 (0.12)
Testes-biased genes sensitive to mutations in meiotic arrest genes but moderately affected by modulo mutation
Mst98Ca	0.003 (0.002)	0.014 (0.000)	0.020 (0.002)	0.236 (0.052)	4.2 (0.002)^*
CG10934	0.030 (0.006)	0.041 (0.000)	0.108 (0.002)	0.294 (0.007)	3.4 (<0.0001)^*
Pros28.1B	0.038 (0.002)	0.052 (0.013)	0.078 (0.019)	0.342 (0.148)	2.9 (0.016)^†
Testes-biased genes sensitive to mutations in modulo but moderately affected in meiotic arrest mutants rye and sa
Ssl	0.081 (0.006)	0.273 (0.012)	0.293 (0.023)	0.041 (0.030)	24.2 (0.0003)^*
Testes-biased genes sensitive to mutations in meiotic arrest genes and modulo
dj	0.000 (0.000)	0.006 (0.000)	0.007 (0.002)	0.113 (0.137)	8.8 (0.008)^*
β(2)Tub	0.040 (0.005)	0.047 (0.002)	0.074 (0.002)	0.131 (0.042)	7.6 (0.0008)^*
fzo	0.003 (0.000)	0.015 (0.003)	0.039 (0.002)	0.084 (0.041)	12.0 (0.0007)^*
dhod	0.002 (0.002)	0.015 (0.005)	0.009 (0.001)	0.088 (0.057)	11.3 (0.0013)^*
Sdic	0.001 (0.000)	0.095 (0.001)	0.048 (0.002)	0.058 (0.052)	17.2 (0.001)^*

Testes were dissected from the achi¹, sa¹, Taf12L^KG00946(rye), and mod⁰⁷⁵⁷⁰ mutants and from the wild type, and transcripts were quantitated by real-time RT-PCR. The amounts of transcripts in mutants relative to the wild type are shown; the gene Rp49 was used as the cDNA template loading reference. Transcripts of the six spermatid-differentiation genes most severely affected in the mod⁰⁷⁵⁷⁰mutant are in boldface type.

*Down-regulation in mod⁰⁷⁵⁷⁰highly significant (P< 0.01).

^†Down-regulation in mod⁰⁷⁵⁷⁰significant (P< 0.05).