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Loss of SOX9, SOX8, and AMH expression by E14.5 in Wt1−/flox; AMH-Cre Sertoli cells. Control testes show normal SOX9 (a and g), SOX8 (b and h), and AMH (c and i) expression in Sertoli cells at E13.5 and E14.5. SOX9 (d), SOX8 (e), and AMH (f) expression was normal in E13.5 Wt1−/flox; AMH-Cre testes but was virtually absent in E14.5 testes, even in those Sertoli cells associated with tubules (asterisks, j–l). sc, Sertoli cells.
