Fig. 7. Histone H3 phosphorylation in Coffin–Lowry cells. Wild-type or Coffin–Lowry immortalized human fibroblasts were serum starved overnight and then stimulated with anisomycin (10 µg/ml, 30 min) or TPA (400 ng/ml, 30 min). (A) Cells were lysed, and histone H3 was extracted from the pellet and analysed for the phosphorylation on Ser10 by immunoblotting. The soluble fraction of the cell lysate was analysed by immunoblotting for (B) phosphorylation of CREB and levels of (C) RSK2 and (D) total ERK1/2 (loading control).