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. 2006 Aug 1;103(32):12039–12044. doi: 10.1073/pnas.0602216103

Fig. 1.

Fig. 1.

Bioinformatic analyses of ≈0.7-kb Mkrn1-like transcripts. (A) BLAST sequence similarity with Mkrn1-p1. The schematic shows the Mkrn1-p1 1.6-kb pseudogene (green) with the 0.7-kb region (yellow) previously claimed to be transcribed (6). The latter sequence (GenBank AF494488) was used as a BLAST query to screen mouse ESTs. Some ESTs diverge after exon 3 (blue dots) and continue into intron 3 (exon 3b). (B) BLAST sequence similarity with Mkrn1 exon 3b. The schematic shows 5′ Mkrn1 highlighting splicing of the exon 3b isoform. Shading indicates the Makorin-1 coding sequence (black), untranslated regions (cyan), and polyadenylation signal (red). BLAST searching identified EST clones containing exon 3b. (C) Summary of mouse dbEST BLAST returns. The total EST numbers matching queries for Mkrn1-p1, Mkrn1-ex3b (0.75-kb), and full-length Mkrn1 (composed of the 0.75-, 1.7-, and 2.9-kb isoforms) are shown.