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. 2006 Aug 1;103(32):12039–12044. doi: 10.1073/pnas.0602216103

Fig. 4.

Fig. 4.

Mkrn1 gene-trapped mice have no overt phenotype. (A) Mkrn1 and gene-trap structures. (Top) Full-length Mkrn1 transcripts span exons 1–8 (symbols as for Fig. 1B). (Middle) The BayGenomics RRB087 gene-trap mutation (s.a., splice acceptor) integrated at a point 735 bp into intron 3 of Mkrn1. (Bottom) The encoded polypeptides from Mkrn1 WT and gene-trapped mRNAs are depicted, with putative functional motifs reverse-shaded. (B) Quantitative RT-PCR of Mkrn1 transcripts. A TaqMan primer set flanking Mkrn1 intron 7 amplified full-length 1.7- and 2.9-kb Mkrn1 transcripts in WT (+/+) and gene-trapped (−/−) brain and kidney. (C) Mkrn1 gene-trapped mice do not have polycystic kidneys or an open-eye phenotype. (Upper) Histological analyses of kidney coronal sections at 7 weeks of age (genotypes as in B) showing renal cortex (C), medulla (M), and medullary pyramid (P). (Lower) Newborn Mkrn1 gene-trapped heterozyogous (+/−) or homozygous (−/−) littermates have closed eyelids (black arrows, the third pup has unpigmented eyes).