Table 2.

rctA acts as DNA to inhibit oriCII function

rctA plasmid in trans (insert description)	Relative copy number of oriCII plasmid pTVC35
	Low [RctB]*	High [RctB]*
pTVC61 (no fragment)	1	1.30 ± 0.20
pTVC43 (rctA ORF →)†	<0.1	0.15 ± 0.03
pTVC44 (rctA ORF ←)	<0.1	0.13 ± 0.04
pTVC45 (rctA ORF →)‡ 2×	NA	<0.1

*RctB is expressed from P_BADrctB in pTVC11 in the presence of either 0.002% (Low) or 0.2% (High) arabinose.

^†The arrow shows the direction of rctA fragment.

^‡pTVC45 has two copies of rctA. It could not transform cells with pTVC35 and low RctB. NA, not applicable.