Table 3.
Rescue of V. cholerae growth inhibition due to excess rctA by transcription of the gene or providing excess RctB
Plasmids (relevant description) | Colony size of transformants |
---|---|
pTVC12 (vector for rctB)/pTVC40 (rctAORF) | No transformants |
pTVC12/pTVC58 (PBADrctA) | Small |
pTVC12/pTVC58 | Large* |
pTVC17 (PlacrctB)/pTVC40 | Small†‡ |
pTVC17/pTVC58 (PBADrctA) | Medium‡ |
pTVC17/pTVC58 | Large*‡ |
*In the presence of 0.2%.
†Plasmids in transformants were partly deleted.
‡Results were same when rctB was induced with 50 μM IPTG.