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Effect of rctA on DNA content and cell size of V. cholerae
| Plasmids in V. cholerae | % arabinose | % (no.) of cells |
||
|---|---|---|---|---|
| Normal | <50% DNA | ≥3× size* | ||
| Vectors (pMLB1109 + pTVC12) | 0 | 90 (795) | 7.8 (69) | 2.3 (20) |
| PBADrctA + vector | 0 | 45 (273) | 46 (276) | 8.7 (52) |
| (pTVC58 + pTVC12) | 0.2 | 90 (744) | 8.7 (72) | 1.2 (10) |
| PBADrctA + PlacrctB (pTVC58 + pTVC17) | 0 | 94 (464) | 5.2 (26) | 1.2 (6) |
*Cells were largely empty in the majority of cases.
