Table 1.

Specificity of interaction between GhCesA1 ZnBD and MET

Yeast cells transformed with plasmids containing the GAL4-binding domain (pBD bait control sequences or CesA1 ZnBD) and the GAL4-activating domain (pAD prey for a clone encoding the MET sequence) were grown on SD medium lacking lysine, tryptophan, and histidine (−L-T-H) or only lysine and tryptophan (−L-T); β-galactosidase assays (LacZ) were carried out on individual yeast colonies. pBD-WT, wild-type fragment of C λ cl repressor, specific interaction control; pBD-MUT, E233K mutant fragment of λ cl repressor, specific interaction control; pLamin C, human lamin C, negative control. pBD-GAL4 Cam, specific interaction control.