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. 2006 Jul 31;103(32):12197–12202. doi: 10.1073/pnas.0603571103

Fig. 1.

Fig. 1.

FAAH expression in Arabidopsis. (A) AtFAAH mRNA transcript levels quantified in seeds, seedlings, and different organs of 6-week-old Arabidopsis plants by quantitative real-time RT-PCR. ACT8 was used to normalize AtFAAH expression levels and plotted relative to transcript levels in inflorescence stems. AtFAAH was expressed in all plant organs with highest levels quantified in 4-d-old seedlings and siliques of 6-week-old plants. Data points represent mean ± SD of triplicates of an experiment. (B) AtFAAH::GUS is expressed strongly in embryos 24–48 h after imbibition and in 4-d-old seedlings. The depletion of total NAEs in germinating seeds (C) correlated strongly with increased FAAH enzyme activity toward NAE12:0 and NAE18:2 (D).