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. 2006 Jul 31;103(32):12197–12202. doi: 10.1073/pnas.0603571103

Fig. 2.

Fig. 2.

Characterization of NAE amidohydrolase activity and NAE sensitivity of AtFAAH knockouts. (A) Reduced NAE amidohydrolase activity in microsomes of knockouts is indicated by the absence of free fatty acid (FFA) peak. The amount of FFA formation was determined by incubating synthetic NAE18:2 and NAE12:0 with microsomes from seedlings. (B) NAE12:0 induced a dose-dependent reduction in seedling development in Arabidopsis wild type as indicated by reduced cotyledon area. The effects of NAE12:0 on Arabidopsis cotyledon area was more pronounced in AtFAAH knockouts. (C) Wild-type seedlings are able to recover from exogenous NAE12:0 2 weeks after germination, whereas AtFAAH knockouts remain severely stunted.