Fig. 3.

Characterization of NAE amidohydrolase activity and NAE sensitivity of AtFAAH overexpressors. (A) Increased NAE amidohydrolase activity in microsomes of three AtFAAH-overexpressing lines. The amount of free fatty acid formation was determined by incubating synthetic NAE18:2, NAE16:0, and NAE12:0 with microsomes from seedlings. Three independent AtFAAH overexpressors were able to sustain hypocotyl (B) and cotyledon (C) expansion despite elevated levels of exogenous NAE12:0. (D) Extended exposure to 500 μM NAE12:0 is strongly inhibitory to vector control and wild-type seedlings. AtFAAH overexpressors, on the other hand, display robust growth despite the high levels and extended exposure to exogenous NAE12:0.