Fig. 1. Surface expression of SHPS-1 and CD47 on WM239a melanoma cells. Cells were incubated with the mAbs SE12B6 to SHPS-1 (A), the mAb CC2C6 to CD47 (B), or the mAb B1D5 to SIRPβ (C). Immunocomplexes were then detected with FITC-conjugated goat pAbs to mouse IgG (thick trace) and flow cytometry. The specific mAbs were replaced by normal mouse IgG as a negative control (thin trace). (D) Whole-cell lysates (20 µg of protein) of WM239a cells, of CHO-Ras cells (a negative control), or of CHO-Ras cells stably expressing human SHPS-1 (CHO-Ras-hSHPS-1 cells; a positive control), were subjected to immunoblot analysis with pAbs to SHPS-1 (αSHPS-1). The positions of SHPS-1 and of molecular size standards are indicated. All data are representative of three independent experiments.