Fig. 4. Effects of SHPS-1 ligands on cell migration in an assay of wound healing. Monolayers of WM239a cells were wounded and then cultured for 0 or 24 h in the presence of control mouse IgG (5 µg/ml), mAb SE12C3 to human SHPS-1 (5 µg/ml), or human CD47-Fc (10 µg/ml), as indicated. Cell migration into the wound was examined by phase-contrast microscopy (original magnification, ×10). Data are representative of a total of three independent experiments.