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. 2006 Aug 7;103(33):12335–12340. doi: 10.1073/pnas.0604849103

Fig. 2.

Fig. 2.

RA inhibits ADAS adipogenesis. (A) ORO staining for ADAS cultured in adipogenic media supplemented with RA and/or BMP-2. Note the dramatic reduction in ORO staining with the addition of RA or RA and BMP-2. Quantification of staining is noted in the upper right corner of each panel. (B) QRT-PCR analysis revealed an increase in adipsin expression for all conditions (∗, P < 0.05; n = 3 for each differentiated ADAS sample). However, a significant decrease in adipsin mRNA transcripts was appreciated with either RA or RA and BMP-2 supplementation relative to plain adipogenic media or adipogenic media with BMP-2 alone (∗∗, P < 0.05). (C) In contrast, alk phos was observed to be significantly up-regulated with RA or RA and BMP-2 (∗, P < 0.05; n = 3 for each differentiated ADAS sample).