Fig. 4.

BMPR-IB expression is dynamic during ADAS osteogenesis and adipogenesis. (A) BMPR-IB transcripts were significantly up-regulated in ADAS undergoing osteogenic differentiation, whereas adipogenesis resulted in a reciprocal decline in BMPR-IB expression (∗, P < 0.05; n = 3 for each sample). In contrast, changes in the expression profile for BMPR-IA and BMPR-II were less dramatic. (B) Western blot analysis revealed that BMPR-IB in undifferentiated ADAS was enhanced at day 7 in the presence of RA or RA with BMP-2 relative to cells cultured in ODM or ODM supplemented with rhBMP-2 alone. (C) Coomassie blue staining demonstrated equivalent loading of protein in each lane.