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Identification of PGE2 (A; m/z 351) and PGE3 (B; m/z 349) by MS/MS in the tumor samples of fat-1 transgenic mice. LC-MS chromatograms showing the relative contents of PGE2 (C) and PGE3 (D) in melanoma and surrounding tissues of fat-1 transgenic and WT mice. Combined samples of three aliquots of tissues (tumor or stroma) from three different animals (fat-1 or WT) were extracted and analyzed for PGE2 and PGE3 by LC-MS/MS.
