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. 2006 Aug 3;103(33):12499–12504. doi: 10.1073/pnas.0605394103

Fig. 4.

Fig. 4.

Effects of PGs (PGE2 and PGE3) on the growth of B16 melanoma cells and PTEN expression. (A) MTT assay of cell proliferation. B16 cells were treated with 1 μM PGE2 or 1 μM PGE3, and viable cells were determined at different time points by MTT assay. (B) Percentage of apoptotic cells after treatment with various concentrations of PGE3 for 72 h, determined by flow cytometry. (C) Western blot showing expression of PTEN. After treatment with 1 μM PGE2 or 1 μM PGE3 for 48 h, cells were harvested, and Western blot was performed to detect PTEN expression. β-Actin was used as control.