Table 1.
Profiles of polyunsaturated n-6 and n-3 fatty acids in stromal and tumor tissues from WT or fat-1 transgenic (fat-1) mice
| PUFAs | Stroma |
Tumor |
||
|---|---|---|---|---|
| WT | fat-1 | WT | fat-1 | |
| 18:2n-6 | 27.5 ± 1.5 | 25.1 ± 1.0 | 12.5 ± 0.8 | 11.5 ± 0.6 |
| 18:3n-3 | 0.1 ± 0.1 | 0.3 ± 0.1* | ND | ND |
| 20:4n-6 | 2.0 ± 0.2 | 1.5 ± 0.1* | 12.8 ± 0.6 | 9.1 ± 0.3* |
| 20:5n-3 | ND | 0.5 ± 0.1* | ND | 0.3 ± 0.1* |
| 22:4n-6 | 0.5 ± 0.1 | 0.3 ± 0.1 | 1.7 ± 0.3 | 1.0 ± 0.2* |
| 22:5n-6 | 1.0 ± 0.2 | 0.4 ± 0.1* | 1.8 ± 0.2 | 0.5 ± 0.2* |
| 22:5n-3 | ND | 0.5 ± 0.2* | ND | 0.7 ± 0.1* |
| 22:6n-3 | 0.4 ± 0.1 | 2.2 ± 0.4* | 0.6 ± 0.1 | 1.3 ± 0.3* |
| n-6/n-3 (total) | 62 | 7.6 | 48 | 9.5 |
| n-6/n-3 (>C20)† | 9 | 0.7 | 27 | 4.6 |
Total lipids of tumor or surrounding tissues were extracted and subjected to analysis of gas chromatography. The values (% of total fatty acids) are means of four separate measurements ± SD.
*Significant difference (P < 0.05) between WT and fat-1.
†Ratio of those n-6/n-3 fatty acids with 20 or more carbons in length, i.e., (20:4n-6 + 22:4n-6 + 22:5n-6)/(20:5n-3 + 22:5n-3 + 22:6n-3).