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Quantitative RT-PCR assays of PTEN, p21, and cyclin D1 expression in WT and fat-1 transgenic mice
| Gene names | C/t values |
ΔC/t | Folds changed | |
|---|---|---|---|---|
| WT* | fat-1 | |||
| GAPDH | 35.2 ± 0.4 | 33.1 ± 0.3 | 2.0 ± 0.7 | — |
| PTEN | 31.3 ± 0.3 | 24.1 ± 0.6 | 7.2 ± 0.9 | ≈32* |
| P21 | 32.0 ± 0.4 | 30.5 ± 0.5 | 1.5 ± 1.0 | NS |
| Cyclin D1 | 35.0 ± 0.4 | 35.5 ± 0.5 | 0.6 ± 0.9 | NS |
Values are means and standard deviations of three separate assays. NS, no significant changes.
*Increase.
