Fig. 6.
CFT073 supernatant affects cell–cell interaction. (A) MG1655F′ biofilm formation in microfermentors with media supplemented with CFT073 supernatant (S.CFT) at times 0, 1, 6 (24 h of culture), and 24 h (48 h of culture). Ø, no addition of S.CFT. (B) GFP-tagged MG1655 F′ inoculated in a flow cell and monitored by confocal microscopy. CFT073 or KS272 supernatants were supplemented after 3 h of culture, and biofilms were grown for 12 h total. (C) Autoaggregation assay with strains that aggregate via different mechanisms: MG1655 F′ (F conjugative pilus expression); MG1655ompR234 (curli overexpression); MG1655ΔoxyR (Ag43 autotransporter adhesin overexpression); and 1094 (cellulose production). Cells were diluted to OD600 = 2 in 3 ml of M63B1 (▴), CFT073 supernatant (·), and ΔkpsD supernatant (
).