Fig. 2.
Regulation of ms-npf expression by the tra-dependent sex determination pathway. (A–C) Expressions of ms-npf in D1 (Left) and in D2 (Center) neurons were examined by immunohistochemistry and in L1-s neurons (Right) by in situ hybridization (hyb). Male-specific D1, D2, and L1-s are indicated by blue arrowheads. (A) WT male. (B) Lack of D1 and D2 neurons in WT female. The single L1-s cell type (which was occasionally observed) is indicated by a green arrowhead. (C) Presence of D1, D2, and L1-s in XX tra1. (D and E) Expression of lacZ in D1 and D2 neurons (arrowheads), in an npf-gal4/UAS-lacZ male (D), was abolished by traF-mediated feminization in the brain of a UAS-traF/+; npf-gal4/UAS-lacZ male (E). [Scale bars, 100 μm (A–C) and 50 μm (D and E).]