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Detection of a T.C mismatch in a 547 bp DNA fragment by using KMnO4 / piperidine assay. The control trace (homoduplex, top) shows no cleavage peak and the mismatched DNA trace (heteroduplex, bottom) displays a strong cleavage peak of the mismatched T base in the 3'HEX sequence. Note consistent background in both traces which is essentially a chemical sequencing trace of T bases allowing confidence that reaction has occurred and a position reference.
